He first sprinted Ishimaru's time on the 40 yard dash but, after hearing Eyeshield's time, he tries again and easily shaves off 0.1 in his second run. The team decided to test his stats for strength and speed. One story introduces a recent 2nd year transfer student who is interested in joining the team. The game splits into four different scenarios such as finding Sena's missing uniform, catching an Eyeshield imposter, or solving 'mysteries' around town via Scooby Doo. Having a 100% completed save will unlock Sena's Devil Bat Ghost which can be ported to its Nintendo DS companion, Eyeshield 21: MAX Devil Power. Post game, regardless of the ending, the player gains access to their earned action scenes, character gallery, team profiles, and the very useful map jump which allows the player to start from any place where they left off. The 'best' endings are given to the player meets all of the greatest conditions and successfully finishes all of the mini games. A handful of endings are 'average' which means that the result from the player's actions ends the story but not to its fullest potential. Most endings are considered 'bad' as they are usually failed attempts resulted from making uncharacteristic choices (one includes Sena quitting the team because he loses interest in football) or losing an action scene.

The game splits into four different stories (with two unlockable after completing the first four) with 107 possible endings. Most of the time, the results are pass or fail, leaving little room for error. Action scenes range from the expected -Sena performing his running cuts, Sakuraba running from his fangirls, etc.- to the bizarre such as using the shine from Yukimitsu's forehead to scare away a pack of dogs or ensuring that Mamori eats 35 cream puffs in 35 seconds. When the player activates certain conditions, they will be treated interactive mini games, or action scenes, that are all completed with different conditions. Sena can also call members from the team with his cell phone, and depending on the scenario, the control may switch to the other character. The player controls Sena based on a selected list of actions. Instead, the game is an interactive text adventure with non moving 2d sprites of characters superimposed over backgrounds from the anime. Despite being a sports game, there are no football matches taking place during the time of the game.

Video eyeshield 21